Fig. 2. Δ264 and Δ27-264 CFTR are primarily located in the ER, but some reaches the Golgi. (A) CFBE41o^- parental cells were plated on coverslips and transfected with Δ264 CFTR-mCherry (red, CFTR). At 48 h post-transfection, the cells were fixed, permeabilized, and stained with DAPI (blue, nucleus) and antibodies against KDEL (green, ER). Close association or co-localization is indicated by a yellow signal. (B) Cells were treated the same way as in (A) but stained with antibodies against Golgin-97 (green, Golgi). The red arrows indicate close association or co-localization between Δ264 CFTR and Golgin-97. (C) CFBE41o^- parental cells were plated on coverslips and transfected with Δ27-264 CFTR-mCherry (red, CFTR). (D) Cells were treated the same way as in (A) but stained with antibodies against Golgin-97 (green, Golgi). The red arrows indicate close association or co-localization between Δ27-264 CFTR and golgin-97. Representative extended-focus images are shown. The data shown were selected from four independent experiments. Representative extended-focus images are shown.